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One-way ANOVA adopted by an LSD check was used for multiple post hoc comparisons of means. The rats in the stress group gained less body weight than the rats in the control group, but there was no statistical difference between the AS, fluoxetine, and model teams. On the other hand, reducing restraint stress to 1 h per day in order to overcome this difficulty leads to a mild stress protocol. On the day after the last habits check, all of the rats had been anesthetized utilizing 10% chloral hydrate (350 mg/kg, intraperitoneal injection) and then decapitated. Treatment with AS (1 g/kg) and fluoxetine (10 mg/kg) resulted in the next level of hippocampal phosphorylated ERK 1/2 than was noticed in the mannequin rats (). The BDNF signaling pathway has been proven to be inhibited in rats subjected to CUMS, resulting in a reduction in hippocampal BDNF, p-ERK 1/2, and p-CREB protein levels. As proven in Figure 6, hippocampal BDNF ranges have been measured at the finish of experiment. As proven in Figure 7, the CUMS procedure resulted in clearly decrease ranges of CREB phosphorylation in the hippocampus than have been observed in the control rats ().