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The procedure contains different mild stressors such as water deprivation for 24 h, food deprivation for 24 h, tail pinch for 1 min (clip used 1 cm near the end of the tail), bodily restraint for 2 h (plastic bottles), cold water for 5 min (5°C), sizzling water for 5 min (45°C), overnight illumination, soiled cage for 24 h (200 mL water and 100 g sawdust mixed collectively), or cage tilted (45°) for 7 h. The stressors have been randomly carried out every day for five weeks except water or food de